The GFP-fusion P2RX4 is an ideal construct to determine the optimal conditions for Accudenz DGU as the liposome-associated protein can be readily monitored by fluorescence.
The shortest linker necessary for activation contained only 13 residues from the SH2-kinase linker; we used this construct to determine the crystal structure described earlier.
We knocked down the expression of Id1 by an siRNA-mediated Id1 lentiviral construct to determine the functional importance of Id1 in EPCs of patients with ovarian cancer,.
The first approach [ 6] focuses on a specific target gene, usually including a transcription assay that uses a reporter-gene construct to determine the specific DNA fragment interacting with p53.
In detail, we used a short hairpin construct to determine the potential of the 5′-radical moiety to derive long distance restraints, which represent valuable parameters in the solution structure determination process.
The act7 intron isolated from Arabidopsis was substituted with the Gladiolus intron in the G1-1 constouct to determine the effect of a dicot intron rather than a non-cereal monocot intron on transient GFP expression and translation in tobacco.
