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1. Construct the sigma points.
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The selection required replacement of the FLO11 ORF with a HIS3 -PEST construct in the Sigma tec1 Δ strain.
Two 35-storey twin towers are projected to be constructed in the Sigma area in Zorilor, while the Florești area will host a complex of three towers with 32 levels each.
Primers used to construct the S. Typhimurium deletion mutant strains were purchased from Sigma-Genosys (Table 1).
Construct the neckband.
Construct the trailer frame.
Construct the tongue.
Construct the rafters.
Construct the game board.
Construct the roof.
Construct the hitch.
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