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Then, contigs were aligned with the reference genome to construct the draft chloroplast genome of each species in Geneious 9.0.5 software (http://www.geneious.com).
An in-house program was developed using BLASTX algorithms by comparing with five green algal lineages and one high plant species to construct the draft transcriptomic database of D. tertiolecta for RNA-Seq analysis and further target gene manipulation.
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Having constructed the draft PMOS measure, it will be important to explore its acceptability and face validity.
The contigs and annotated gene sets of T. urartu and Ae. tauschii have been useful for constructing the draft genome of common wheat [ 24].
Using the alignment order of bovine Y chromosome, we constructed the draft assembly of wild goat chromosome Y (see the Additional file 1: Supplementary Methods 3.1 for further details).
There is also a possibility that some of the FLNC reads in G4 and G5 may come from divergent variety-specific genes since the elite common wheat variety used here (i.e., Xiaoyan 81) may differ from the land race CS used for constructing the draft genome sequence.
By combining the first-generation virtual genome map presented in this paper with the Ensembl annotation [ 13] of the initial (Meug_1.0) tammar wallaby assembly, it is now possible to construct the first draft chromosome assembly for the tammar wallaby.
Therefore no preliminary models were formulated and only double items were removed to construct the first draft of the questionnaire in order to lose as little information as possible.
This might be because, since we used chicken genome as reference to construct the black grouse draft genome for the heterozygous nucleotide sites, the choice of the nucleotides could be biased towards the chicken reference genome.
JU constructed the first draft and conducted the literature search.
JE constructed the first draft of the manuscript with subsequent inputs and revisions from OB and PK.
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