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To determine the time course of expression of each construct, the amount of applied protein was adjusted in proportion to the total amount of cellular protein in each sample, starting with 25 µg of cellular protein at 0 h post-transfection.
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This construct - the primary insurance amount doesn't exist until it's triggered by a retirement benefit application -- lets Social Security claim to have one formula for spousal benefits.
The human and mouse fibroblasts were transiently transfected with the GFP-hGH reporter construct and the amount of this reporter molecule in the Golgi area was analysed at 0, 15 and 30 minutes after AP21998 ligand addition.
The bridge over the canal that currently uses PA 405 was constructed in 1854 at a cost of $27,000 (1854 USD), nine times the amount to construct the canal.
However, EZBC used Amplitude Quadtree Q k [l](i, j) and two link lists (LIN and LSP) to finish the coding process and utilized the significance of the neighbor nodes and the node in parent subband to construct the context, so a large amount of memory was required to store Amplitude Quadtree Q k [l](i, j) and two link lists.
To construct the selective DNA pools, equal amounts of the amplified genomic fragments from each bull were assembled into the respective high and low EBV (SCS) pools.
However, we have found through experimental testing that while these protocols can increase network throughput in carefully constructed scenarios, the amount of throughput gain realized in a network with random node placement is low.
In order to gain more insight into the MEP pathway of B. subtilis, conditional knockouts of the MEP pathway genes of B. subtilis were constructed, then the amount of emitted isoprene was analyzed.
Based on this work, Milsom and colleagues [ 41] first tested two constructs, pHoxB4-2A-GFP and pMGMT-2A-GFP, coexpressing HoxB4 and MGMT (O-methylguanine-DNA-methyl-transferase, a transgene that conveys chemoresistance) with GFP and noted that in cells transfected with these constructs the amount of uncleaved [MGMT-F2A24-GFP] polyprotein was far greater than that of [HoxB4-F2A24-GFP].
"It takes a significant amount of time to construct the set ups.
RNA isolated of three breast reductions, mixed in equal amounts, was used to construct the normal skin expression profile library.
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