This method analyzes and quantifies side-to-side asymmetry of CBF and represents relative differences in a construct termed a relative difference map (RDM).
A guiding principle in built environment design for sustainability and livability is a latent construct termed Sense of Place (SOP), which leads visitors to perceive and associate a strong identity or character with a particular location.
Real-time fluorescence activity assays using an optimized sensor construct, termed FAKtide-S2, are highly reproducible (Z' = 0.91) and are capable of detecting as little as 1 nM recombinant FAK.
Based on recent directed evolution of P450 2B1, six P450 2B11 mutants at three positions were created in an N-terminal modified construct termed P450 2B11dH and characterized for enzyme catalysis using five substrates.
Consequently, a third construct, termed C34V, which contained a FLAG® epitope (DYKDDDDKDG) located just downstream to the TRS was tested (C34V; Fig. 1C).
Surprisingly, this construct termed 'Rv0113 trunc' produced an insoluble truncated protein (Fig. 5A, column B), although the DNA was full-length (data not shown).
In order to investigate if this sequence was targeting chimeric proteins for degradation through a lysosomal pathway, we assessed the localization of the GFP-CINCCKVL construct, termed GFP-8, in the presence of lysosomal inhibitors.
This construct, termed pPK25, served as the parental plasmid for all puf-8 deletion constructs.
An EXO overexpression construct (termed 35S::EXOga) was transformed into the exo mutant.
The resulting construct, termed pET21-LasRLBD, was transformed in Escherichia coli BL21 DE3 pLysS cells for protein expression.
