Sentence examples for construct substrates from inspiring English sources

The oligonucleotides used to construct substrates for single-nucleotide gap-filling reactions were the 5′-6-carboxyfluorescein labeled primer (5′-CTG CAG CTG ATG CGC-3′), the downstream oligonucleotide (5′-GTA CGG ATC CCC GGG TAC-3′), and the template (3′-GAC GTC GAC TAC GCG GCA TGC CTA GGG GCC CAT G-5′, where the templating G in the gap is underlined).

The oligonucleotides used to construct substrates to assay removal of a 5′-dRP group were the primer (5′-CAT ATC CGT GTC GCC CTC-3′), the downstream oligonucleotide (5′-U ATT CCG ATA GTG ACT ACA-6- carboxyfluorescein-3′), and the template (3′-GTA TAG GCA CAG CGG GAG TAA GGC TAT CAC TGA TGT-5′).

However, using recombinant WT C100Flag, 3xK-C100Flag, and K28E-C100Flag (K28 is equivalent to K624E in the C-100 construct) as substrates in in vitro γ-secretase assays, we were able to detect AICD and ε-cleavage site utilization.

Thus, more efforts are being made to construct broad substrate-range mini-cellulosomes (Fontes and Gilbert 2010) and multiple scaffoldin units in larger cellulosomes (Fan et al. 2012) for biofuel production at the industrial scale (Alper and Stephanopoulos 2009).

For this we used a heavily BODIPY dye labeled casein construct (Enzcheck substrate).

Figures 2A and 4A illustrate the constructed DNA substrates where the upstream and downstream oligonucleotides are 5′-phoshphorylated with [γP] ATP or unlabeled ATP, respectively.

We have constructed DNA substrates that correspond to the site 1 and site 2 integration products for the addition of spacer 3 into the CRISPR array (new Figure 9).

In order to monitor specific NS3 proteolytic activity in Tet induced cells, we constructed a substrate-encoding plasmid that encodes for a modification of our previously described polypeptide which serves as a substrate for proteolysis by the NS3 protease [33].