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To investigate the role of MUC1-CD in mammary development, we generated transgenic mice that overexpress GFP-tagged human MUC1-CD (72 AA) cDNA under the control of an MMTV promoter [28],[29] in the mammary gland with the construct shown in Fig. 1A.
This exercise reinforces the energy construct shown in the small blue box in Figure 1.
Z.H. made the pBI LAT52 SLF 2 CTD) :GFP construct shown in Fig. 1A and performed some of the plant transformation experiments.
The experiments are well performed, and the reasoning behind the experiments is well explained, but the experiments all rely on a particular DNA construct, shown in Figure 1.
The discrepancy between the lack of toxicity of this 'S14A' construct and the toxic S11A construct shown here might derive in part from this lack of amino terminal sequences that may positively regulate aggregation (59).
To determine if we could achieve higher levels of recombinant protein accumulation by fusing a recombinant protein with a highly expressed endogenous protein, we assembled the chimeric gene construct shown in Figure 1B.
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No construct showed negative correlation with the first principal component.
For some receptors, the Nt_BRIL construct showed a high expression level, while the ICL3_BRIL construct showed low expression (Table 6).
This construct shows significantly reduced binding to human netrin-1 (Fig. 1B).
This cell construct showed high cell survival and minimal cytotoxicity in vitro.
Mice expressing the CAG200 construct showed normal weight gain and did not have an overt phenotype.
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