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That construct serves to limit people's images of themselves". Puryear began exhibiting his sculpture and completed his first major outdoor sculpture for Artpark, in Lewiston, N.Y., in 1977, but his career was set back by a fire in his studio that destroyed much of his early work and his documentation.
While the KAB aims to explain behavioral changes and the TAM seeks to explain technology use, our construct serves another purpose.
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The site-directed mutagenesis construct served as template in the primer extension reaction that employed a fluorophore labeled primer in close proximity to the mutation.
Lysate from mock-treated cells, transformed with the empty expression construct, served as negative controls for analysis.
The Tat-FLAG construct served as template for site-directed mutagenesis (Stratagene) to generate the F38A mutation.
The SV40 promoter reporter construct served as positive control.
This construct served as the standard for comparison with the fusion proteins described below.
This construct served as the parent cloning vector for cassette-based mutagenesis.
This construct served as an additional control next to the expression of TVAG_386160::HA.
A constitutive Renilla luciferase construct served as an internal control for normalizing transfection efficiencies, cell viability and cell numbers.
The Y258 strain with an integrated URA3 selectable marker that was not transformed with the tagged construct served as a minus protein control for non-specific binding.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com