However, complex interaction between base rupture, onset of significant cycling, creep, and fatigue dictates the upper bound on feasible ramp rates, as a result it is possible to construct screening curves based on the effective elastic stress intensity range.
Here we describe CoESPRIT, a library scale construct screening methodology that incorporates co-expression of bait proteins into our previously reported protocol for identifying soluble sub-constructs of a target protein [27], [28].
To accelerate construct screening we further inserted a ccdB promoter and gene cassette, amplified from pDONR211 (Invitrogen, Grand Island, NY), inside the multi-cloning site.
Therefore, it is important to identify thyroid hormone-disrupting compounds (THDCs) and to construct screening methodologies to be included in monitoring programs [ 2].
We could overcome the recalcitrance of holo-AMPK to crystallization in the absence of pharmacological activators by extensive construct screening and by altering three flexible solvent-exposed side chains (E471G/E474A/K476A) at the N-terminus of the ST-loop of the α-subunit (see Materials and Methods and Figure 2A).
When Iran has struck foreign targets, observers note, it has been through trusted local proxies, like Hezbollah, which have allowed Tehran to operate behind a carefully constructed screen of deniability.
Laurent Bouhnik's "24 Hours in the Life of a Woman," a handsome, elaborately constructed screen adaptation of a story by the Austrian novelist Stefan Zweig, wanders back and forth in time without defining a theme or finding an emotional center.
"The sonnets," he writes, "are a cunning sequence of beautiful locked boxes to which there are no keys, an exquisitely constructed screen behind which it is virtually impossible to venture with any confidence".
Rooms can be divided into different spaces by constructing screens of Algues or Clouds, and then dismantling them.
First, a case library consisting of 676 clinical cases, 56.95% of which had been diagnosed with PM and 43.05% of which had been diagnosed with PTTH, was constructed, screened by a three-member panel, and weighted by engineers.
