Sentence examples for construct reporter from inspiring English sources

To construct reporter plasmids, promoters of putative target genes of OsSMF1 were amplified from Oryza sativa cv.

To construct reporter plasmids containing N-termini of a rifin (PFA0745w), a stevor (PFC1105w), and a predicted P. berghei exported protein (PB402722.00.0, Pb), corresponding regions of these genes were amplified from P. falciparum cDNA or P. berghei genomic DNA using Phusion DNA polymerase and primers (Rifin: 51 aa, RifF/RifR; Stevor: 63 aa, SteF/SteR; Pb, 71 amino acids, PBpredF/PBpredR).

To construct reporter vectors carrying the mPer2 promoter, the mPer2 promoter fragment (−279 to +112 bp, where +1 indicates the putative transcription start site) was PCR-amplified from C57BL/6J mouse genomic, and cloned into the NheI/XhoI site of pGL3 Basic (Promega).

Promoter constructs were generated using a pGL3-Promoter (pGL3) luciferase construct reporter vector (Promega, Madison, WI), as described previously.

In the case where inverse PCR was used to construct reporter vectors, whole plasmids were PCR amplified using Phusion DNA polymerase (NEB) along with multiple operator containing oligonucleotides.

Further truncation of the promoter produced progressive decreases in promoter transcriptional activity, such that the activity of the 78 bp construct reporter activity was 50% lower than the 1706 mTfam construct during successive days of differentiation.

Finally the high responsiveness of the constructed reporter supported the hypothesis that GPD2 expression was directly connected to the cell need to oxidize NADH via glycerol formation.

Taken together, the constructed reporter phage phiV10lux could be applied as a powerful tool for rapid and sensitive detection of live E. coli O157 H7 in foods.

To that end we constructed reporter plasmids with serial deletions of the promoter.

So constructed reporter cells were encapsulated in hollow fibers and the fibers were implanted subcutaneously into athymic, nude mice.

To determine whether CDH1 is a direct miR-199a-5p miR-199a-5p miR-199a-5preportargetnstructs using weld-type or mutant constructed freporter.