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Isolated ESS3b does not contain any detectable secondary structure as determined by 1D H NMR spectroscopy (not shown); therefore, thermodynamic measurements using this construct report primarily on sequence determinants of binding.
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Although the initial construct reported [NADH] × [H+] / [NAD+], its pH sensitivity was eliminated by mutagenesis.
Unlike clinically tested CD19-CARs, which contained either CD28 or 4-1BB co-stimulatory endodomains, the GPC3-CAR construct reported in these studies contained both CD28 and 4-1BB endodomains (Gao H et al., 2014).
However, it is interesting to note that comparison of our raw data to those of Tabaie et al. revealed that the locked transsacral screw construct reported by Tabaie et al. has a significantly greater load to failure than our two fixation constructs or the short iliosacral construct tested by Tabaie et al. (Table 5).
For control, the proteolysis of PrPQ218K was assessed, which is a construct reported to be a dominant negative of PrPSc replication [40].
PpHPL was PCR-amplified from the construct reported before [ 18].
Analysis of an MtSCP1 promoter-GFP fusion construct in M. truncatula transgenic roots indicated that the construct reported the presence of the AM fungus in the cortex [ 36].
These results are similar to what was observed on replication of a S-cdG construct reported earlier, and we conclude that pol V (UmuD′2C) is required for replication of both S-cdA and S-cdG.
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WASHINGTON -- Central Intelligence Agency Director John Brennan approved his agency's search of a walled-off computer network being used by the Senate Intelligence Committee to construct a report on the agency's torture program, a CIA Accountability Review Board has found.
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