Sentence examples for construct prepared from inspiring English sources
To analyze cardiomyogenesis in conditions of endogenous OT-GKR production in stem cells, the OT-GKR-IRES-EGFP construct (prepared as reported previously) [15] was transfected into EC P19 cells with a FuGENE 6 kit (Cat. No. 11814443001, Hoffmann-La Roche Limited, Mississauga, ON, Canada).
In order to gain preliminary insight into the genes regulating HA synthesis in this system, a differential gene array analysis was conducted in which the construct prepared in the presence of 200 μg/mL HC and 0.2 nM T3 was compared to the normal construct (0.4 μg/mL HC and 20 pM T3).
Following Sarah Palin's videotaped statement today, let there be no doubt about her total lack of seriousness -- a character trait, in fact, that's utterly dwarfed by her chronic inability to construct prepared, teleprompter-presented remarks without virtually choking on her own tongue.
Amounts of construct (prepared as described in [26]) per well were 30 ng of human TLR2, TLR4 (co-expressing MD-2) or TLR5 (Invivogen), 30 ng of CD14, 20 ng of renilla luciferase-reporter construct and 10 ng of firefly luciferase-reporter construct driven by the NF-κB dependent E-selectin promoter (pELAM).
Porous resorbable polylactic acid (PLA)/cloisite clay nanocomposite constructs prepared using scCO2 processing exhibit a 2.5-fold increase in compressive strength compared with pure polymer constructs.
As a result, gel-cell constructs prepared with HLT2, cultured under static in vitro conditions, contained more GAG and type II collagen resulting in mechanically superior constructs.
These engineered vascular constructs, prepared from doxycycline-inducible inbred siPSCs, offer new opportunities for preclinical investigation of autologous human iPSC-based vascular tissues for patient treatment.
Prolonged pathway activation correlated with enhanced contractile function of vascular constructs prepared from hair follicle mesenchymal stem cells or bone marrow derived smooth muscle cells.
The strength was also considerably higher than the values reported for polymeric, bioactive glass ceramic and hydroxyapatite constructs prepared by the same technique and with the equivalent level of porosity.
However, since constructs prepared ex vivo lack the multitude of cues present in the in vivo microenvironment, cells often need to be supplied with external biological and physical stimuli to coax them toward targeted tissue functions.
Culturing cell hydrogel constructs prepared from HA185k-SH with a DS of 28 and cross-linked with PEG5k-4VS for 3 weeks in vitro revealed that the cells were viable and that cell division took place.
