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The entire process of deletion construct preparation can be accomplished in just 2 days.
When considering the stiffness, we did not find differences between the three groups and this can be explained because the stiffness should be correlated more with the viscoelastic properties of the tendon itself rather than with the construct preparation.
The DY380 recombination competent E. coli strain was used in construct preparation, allowing direct recombination of sub-cloned fragments to generate the final targeting construct.
According to the established experimental scheme, it takes less than one year to obtain potato plants with cisgenic R gene stacks from the R gene construct preparation to the functional validation of the resulting cisgenic plants by performing DLAs.
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In the months since, all of the leaks and all of the tweets warning that there would be no indictment for Darren Wilson – that instead there would be black "violence" and a perpetual "state of emergency" – have served as constructed preparations to manage our disappointment, for the big reveal that our criminal justice system was still as broken as it ever was.
ShRNAs constructs, preparation of viruses and cell transduction have been described previously [ 24].
We would like to thank Francesco Maione and Francesca Russo for participation in constructs preparation.
In response to the presence of a truncated fragment smaller than the catalytic domain construct in preparations of ColT, two shorter variants were cloned to identify an even smaller active catalytic domain.
A large scale model of our site and associated landscape had been constructed in preparation for the testing.
To her, each sequence was constructed of preparation, recovery, and subject — that is, of the act itself.
In this platform, optimum and simple electrochemical conditions were first constructed for preparation of Fe3O4 and γ-Fe2O3 nanoparticles in both ethanol and aqueous mediums, and monodispersed nanoparticles with superparamagnetic properties were prepared.
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