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Taken together, our artificial PLGA construct permits NSCs to differentiate toward neurons, establish connections and exhibit synaptic activities.
Taken together, although our artificial neural construct permits grafted cells to differentiate into neuronal phenotype, synaptogenesis, axonal regeneration and partial locomotor function recovery, the limited capacity for corticospinal tract axonal regeneration may affect its potential therapy in spinal cord injury.
This construct permits robust Tet-regulated p16 expression in cultured cells (Mitra et al., 1999; Dai & Enders, 2000).
Additionally, the CommandSet construct permits the ability to iterate sequentially or concurrently over a subset of the elements of a workflow encapsulated in a CommandSet.
As PA-GFP fluorescence is low until activation by brief high intensity laser stimulation, this construct permits selective activation of small regions of cytosol containing Rab7-positive vesicles.
The fact that the SBE RNAi construct permits simultaneous targeting of three independent SBE genes, is of particular practical importance in breeding.
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The resulting recombinant GRA5-pRSET B construct permitted expression of an N-terminally polyhistidine- (His-) tagged rGRA5 (amino acid residues 26 120), lacking its putative N-terminal signal sequence.
A cohort of genetically identical flies possessing the GeneSwitch Elav driver (GSElav) and a construct permitting overexpression of dSir2 were randomly assigned to three different conditions: (i) high calorie food with EtOH diluent; (ii) low calorie food with EtOH diluent; and (iii) high calorie food with RU486.
To operate the helicon RF ion source with hydrogen/helium plasma a magnetic system with circular permanent magnets was designed and constructed, permitting a generation of a longitudinal magnetic field Bz ∼ 100 G along the length of the RF antenna and of a longitudinal magnetic field ∼1000 G with effective field length of about 10 cm to confine and transfer the plasma to the extraction system.
Thirdly, a FLAG tag fused to the N terminus of the bait (importin α1 or PB1 C-terminal sub-constructs) permits its identification after the IMAC purification of the library protein.
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