Sentence examples for construct obtained from from inspiring English sources

On the other hand, inductive methods base item development on qualitative information regarding a construct obtained from opinions gathered from the target population e.g., focus groups, interviews, expert panels, and qualitative exploratory research methodologies (Kapuscinski and Masters 2010).

We examine the specific requirement of Ppt1 in neurons by driving the expression of Ppt1-RNAi in strains containing the ds RNA-GD3286 cobtained (obtained from the Vienna Drosophila RNAi Center [24]); and the dsRNA-JF01972 construct from the TriP-1 collection (Bloomington Drosophila Stock Center) using various neural-specific Gal4 drivers (Bloomington Drosophila Stock Center).

The p53 luciferase and SIRT1 constructs were obtained from Addgene (Cambridge, MA, USA) and NF- κB luciferase construct obtained from Clontech (Madison, WI, USA).

Using the purified α-fodrin GST protein encoded by the cDNA construct obtained from Dr Hayashi, we developed an ELISA (hereafter referred to as the Nijmegen ELISA) that was used for the analysis of SjS and control sera.

Knockdowns in S2 cells were done using the following constructs: an H1 construct obtained from [ 11] and HMGD1 PCR products were obtained from the Drosophila RNAi screening center (DSRC).

The primers used for the construction of the replacement PCR constructs (obtained from biomers.net) are listed in Table 2. Homology to the hexo-/glucokinase genes, bold; homology to kanMX cassette, italic.

Lentiviral shRNA library composed by 3 5 shRNA for each gene was enriched for human kinases and phosphatases by rearraying LKO.1 shRNA constructs obtained from the RNAi Consortium (TRC) (Broad Institute, MA, USA).

To further investigate which portion of the C-terminal region of CENP-C can mediate the interaction observed with CENP-A and histone H3, we performed a co-immunoprecipitation assay using FLAG tagged histones co-expressed along with the HA-CENP-C constructs obtained from the partitioning of C-terminal region (Figure 7).

pMT- lrrk-9myc was transfected along with pAC-Gal4 and pUASp-Rab YFP constructs (obtained from Matthew Scott) as indicated.

The SMAD4 cDNA overexpression construct was obtained from Origene, and the SMAD2 cDNA (HsCD00001922) overexpression construct [ 65] was obtained from DNASU.

The myc-ubiquitin construct was a gift from Dr R. Kopito, and the GFP-Hsp70 construct was obtained from Dr H. Kampinga.