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We explored the impact of trial quality on effect estimates and used trial sequential analysis to quantify required information sizes and construct monitoring boundaries to assess the risk of random error affecting the cumulative estimate.
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A survey conducted on about 70 of these reservoirs provided considerable information on their pathologies, incidents and even accidents and served as a basis for writing a set of guidelines for studying, designing, constructing, monitoring and rehabilitating.
30– 32 We therefore constructed monitoring boundaries to test for statistical significance in the meta-analysis including all trials up to year 1998, up to year 1999, and up to year 2004, albeit no new trials were published in 1999.
The 2 11 and A10 Clones were selected as highly active clones for these respective constructs monitoring EGFP expression upon the control of heat shock or hyperoxyde stimulation prior to the experiments on chip.
The firefly luciferase reporter construct for monitoring IFN-β promoter activation (p125Luc) was kindly provided by Takashi Fujita, Kyoto University, Japan [48].
(A schematic of the use of a pHluorin construct in monitoring synaptic activity is given in Figure S2. See also Materials and Methods).
The firefly constructs monitor changes in the activity of a key transcription factor which is a downstream target of a particular signaling pathway.
We used a GPI-linked pHluorin2 (Caras et al., 1987; Miesenbock et al., 1998) to monitor extracellular pH and an untagged cytoplasmic pHluorin2 construct to monitor intracellular pH (Fig. 6A).
Transgenic mice carrying these constructs were made and expression of the construct was monitored by staining for β-galactosidase activity.
pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level.
H1299 cells were transfected with the Pig3 promoter reporter construct to monitor p73 activity and BAG-1 and p73 were depleted by siRNA.
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