Sentence examples for construct modification from inspiring English sources

However, in the context of the de-localized Pc2 2-531) construct, modification by SUMO1 of both Pc2 and CtBP was reduced by these point mutations (Figure 5C).

Our work contributes to the understanding of the factors necessary for the successful expression and purification of P2X receptors, highlights the need for construct modification to stabilize trimers (in the case of hP2X4) and demonstrates that DdP2XA is both an interesting and ideal candidate with which to pursue higher-resolution structural studies.

Success with zfP2X4.1 was dependent upon three main factors: (i) screening of many different receptor constructs to find those which formed the most stable trimers in detergent solution [7]; (ii) use of the Sf9 insect cell expression system; and (iii) significant construct modification to achieve well-diffracting crystals.

The extensive construct modification altered ion channel function; peak currents were reduced, channel closure after removal of agonist was delayed, and agonist potency was increased [6], implying that the structure may not represent a native conformation, but rather an artificially stabilized, closed state.

For each miRNA construct three modification PCRs were performed with primers G-4368+II G-4368+II I+IVG-4369 on pNW55 andtemplate, yIIlding fragments of 256, 87 and 259 bp lengths, respectively.

An unconstrained violation is one in which all attempts to construct meaningful modifications of the second law are also invalidated.

Secondly, we generated a full-length HIV-1 construct containing modifications at the -1 frameshift slippery sequence and the immediate downstream RNA pseudoknot that regulates Gag-Pol expression (non-frameshift HIV-1 or HIVNFS).

The retention time prediction model was constructed by modification from past respectable knowledge of retention modeling against ternary solvent strength changes.

We attempted several modifications of the construct to change the level of phoP expression, similar to the strategy we used to improve the fur construct, but none of the changes significantly improved the immunogenicity profile [28].

The main aim in this study, however, was not to estimate the prevalence of a disease, but to construct a valid modification of the CCI.

(A ) Flag-H2be transgenic construct, generated through modification of a BAC containing the H2be genomic region by insertion of a FLAG-encoding sequence immediately upstream of the H2be CDS.