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To this end, a Cd146 construct lacking the KKGK motif (coding Cd146-∆KKGK) or a Moesin construct lacking the actin-binding domain (coding Moesin-∆ABD) was transfected into 10T1/2 cells.
We further show that an ND1 construct lacking D2 but including the linker between D1 and D2 is catalytically active, resolving a conflict in the literature.
The DNA vaccine construct lacking both the SS and the TD domain and the vaccine having only the SS generated lower antibody responses, compared to the rGVRt construct.
A construct lacking all 8 amino acids was unable to rescue the tre1sctt defect (Figure 3).
This procedure produced a WD construct lacking part of the F-box and conjugated to FLAG.
The luciferase construct lacking the PPRE and the Renilla constructs were purchased from Promega.
The decreased phosphorylation of AKT deletion construct lacking PH domain could be due to poor binding with activated Ack1.
In agreement with previous studies, the Head1/Neck3 construct (lacking the conventional neck domain) shows clear defects in processivity.
To rule out shRNA off target effects, we used a previously described β-TrCP dominant negative construct lacking the F-box domain [33].
To determine whether Oto's modification of Wnt3a involves C77, we tested a Wnt3a deletion construct lacking the palmitoylated residue (Wnt3aΔ31 107).
Surprisingly, when miR-137 was co-transfected with a reporter construct lacking the 3'UTR sequence of Mitf, miR-137 downregulated luciferase expression to 27% (Fig. 3B).
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