Sentence examples for construct into one from inspiring English sources

In the near future one only has to clone a cDNA or validated shRNA construct into one of these vectors and introduce that into the GEMM-ESC clone of choice after which an experimental cohort can be produced.

In 2 the dysprosium centers were constructed into one dimensional chain by OCO bridges from pydc ligand bridges and these chains were linked by oxalate bridges to form sheets and different sheets were connected by copper coordination planes.

Out of this ORF candidate, we found 31 ESTs as primary sequences that could be constructed into one potential novel gene in silico (Table 1, Fig. 1).

Multiplex targeting was performed as described above using pCAS plasmids containing more than one sgRNA expression construct cloned into one of the restriction sites by ligation dependent cloning.

These have involved both multiple integrations of one construct into different locations [ 75] or integration of multiple constructs into one site on the X chromosome [ 13].

The animals were allowed to settle for 1 week prior to the subcutaneous implantation of one construct into each flank.

Aggregation of ES cells heterozygous for the targeting construct into 8-cell embryos produced one viable and fertile male mouse with an estimated high percentage of chimerism.

We coinjected these constructs into one cell at the late two-cell stage to restrict the effect to half of the embryo.

We infiltrated the SlMKK2DD and SlMKK4DD constructs into one side of the N. benthamiana leaves for transient expression and then inoculated the opposite side of the leaves with spore suspension of B. cinerea 48 hr after infiltration.

This construct was injected together with a corresponding sgRNA expressing construct into Cas9 expressing embryos.

To resolve the changes in RGC dendritic morphology in greater detail, we mosaically labeled individual RGCs by coinjecting Ath5 Gal4, UAS GFP and UAS:tdTomato DNA constructs into one-cell-stage embryos.