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Importantly, however, the expression of an Aplip-AxxA1 cDNA construct (integrated at the same chromosomal integration site as the control construct; expression and axonal presence confirmed with a newly generated Aplip1 Ab; not shown) could no longer rescue the RBP/BRP accumulation phenotype.
Our work shows a new way to construct integrated cathode for application in LIBs.
In addition, as the demand for a holistic understanding of environmental systems increases, access to external model components is necessary in order to construct integrated models.
The BamH I Bgl II fragment from plasmid pGAPZA*-Z was then subcloned into the BamH I restriction site of plasmid pGAPZA-W and the resulting construct integrated into P. pastoris Pp-EBIL DNA genome by recombination events.
This system was tested in maize using the pat selectable marker gene, whereby up to 30% of the plants regenerated on Bialaphos-containing medium were observed to have the donor construct integrated into the target locus.
The situation faced by students using the Web for research is similar to that faced by history students learning to use primary and secondary sources: They construct integrated representations from multiple documents of varying quality.
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Quality of life (QoL) is a multidimensional construct integrating an individual's subjective perceptions of physical, social, emotional and cognitive functioning [ 13].
Our work displays a new insight in constructing integrated cathode for application in high-performance LIBs.
This auxiliary device was designed, constructed, integrated and tested on a gasoline engine.
Vacuum structure layout of fan blade was constructed integrating the major load transfer paths in each design proposal.
Iso-rate contour plots are constructed integrating the microbial and enzymatic kinetics for the combined T and P treatments.
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