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The PrRP-Cre targeting vector contained an IRES-Cre transgene inserted just downstream of the endogenous STOP codon in PrRP exon 2. The LSL- PrRP targeting construct incorporated loxP sites flanking a transcription STOP cassette just upstream the endogenous PrRP gene and a neomycin-resistance cassette.
Consistent with these predictions, we found that animals expressing the dominant negative AMPK construct incorporated the dye at a higher level (62.2%) compared to animals with wild-type AMPK function (37.7%).
The first construct incorporated the C-terminal 25 amino acids from the human CA25 antigen on to the C-terminus of INV.
MSCs seeded onto a collagen-type-I construct incorporated into healing tendons that subsequently exhibited greater load-related structural and material properties than unseeded constructs.
In order to analyze the constructs in a more in vivo-like setting, we turned to the Shh-Light2 (Shh-L2) cells that have a Gli-inducible luciferase reporter construct incorporated into the genome.
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Multimorbidity was examined using chronic condition counts and a clinical construct that incorporated conditions involving multiple body systems/specialties.
In our studies, we evaluated and found nonlinear concentration effects and used the information for ICG liposome admixtures related to ICG lipid binding and fluorescence enhancement to fine-tune an optimal composition to construct ICG incorporated completely into liposome membranes.
Constructs incorporated either the P MDH1 (KmP MDH1 ) (Additional file 1: Figure S4) or P ACO1 (KmP ACO1 ) (Additional file 1: Figure S5) promoters from K. marxianus.
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage.
These constructs incorporated a constitutive promoter, PN25, driving the expression of the SA, and HRs flanking the flu gene of E. coli K-12 strain MG1655.
These constructs incorporated 1651, 284, 256 or 240bp of the AtMYB80 5'UTR sequence (relative to the ATG translational start codon) into the pBI vector and were transformed into the wild-type Arabidopsis.
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