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Yet, each ERE sequence, when cloned as a single copy, or when engineered as two tandem copies of the ERE-containing sequence, was not capable of activating a luciferase reporter construct in response to E2.
To experimentally test the prediction that the MURE1 and MURE2 sites are involved in mtUPR, we created mutations in these sites in the yme1l1 promoter and tested the effect on the inducibility of a promoter-luciferase construct in response to mtUPR.
In contrast, no significant activation was observed using a pXK-5-2 construct in response to 20 μM iAs.
In addition, recent in vivo work in E. coli shows that the region 162 nucleotides upstream of rpsB controls an rpsB-lacZ fusion construct in response to exogenous S2 added in trans [ 62].
As an initial screen for ligand specificity and efficacy of the expression system, we measured the luciferase activity produced by cells co-transfected with each of the LBD-GAL4 constructs and the luciferase reporter construct in response to hormone treatments at one concentration, 10-7 M. Normalized values were expressed as fold change over vehicle (ethanol) treatment.
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And for it to make sense, it must be an identity constructed in response to facts outside oneself, things that are beyond one's own choices".
Constructed in response to the disaster that was the Iraq War, the Obama doctrine abjures direct U.S. military intervention in countries that don't represent a direct security threat to the United States, such as Syria.
Urban rail transit system in China has been rapidly constructed in response to the effects of urbanization, such as severe urban congestion and excessive air pollution.
We propose that corporate governance of foreign subsidiaries in a transnational corporation should be constructed in response to different levels of agency problems associated with varying strategic roles of foreign subsidiaries.
It has been argued that they could have been defensive sites constructed in response to invasion from continental Europe, built by invaders, or a military reaction to social tensions caused by an increasing population and resulting pressure on agriculture.
It has been argued that they could have been military sites constructed in response to invasion from continental Europe, sites built by invaders, or a military reaction to social tensions caused by an increasing population and consequent pressure on agriculture.
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