Connections are constructed in full scale.
The specimens were constructed in full scale and were able to simulate the real SC members of existing nuclear power plants.
The main cabin of the Orion ship was constructed in full specifically for this episode while the bridge was only a single wall as it was only seen on screen as a view-screen background.
Implantation of a chondrocytes/GCH-chitosan cryogel construct in a full-thickness articular cartilage defect regenerates cartilage with positive stainings for GAGs and COL II and an elastic modulus similar to the native cartilage.
To generate transgenic rice plants overexpressing an exo-glucanase gene, we made a binary vector construct in which rice full-length cDNA (AK108835) for exo-glucanase was placed between a maize ubiquitin promoter and a terminator of a nopaline synthase gene in a sense orientation (Figure 1).
A positive control was ARF5M construct, in which a full-size ARF5M cDNA was subcloned into the GAL4 expression vector [ 53].
The computational models were constructed in the full 'ODE' (ordinary differential equation) form and solved in Matlab version R2007a (The Mathworks Inc).
These cell lines were transfected with a construct in which the human full-length CAR cDNA is expressed under control of the CMV promoter in a pcDNA3.1 expression vector ('hCARpcDNA3.1'; a kind gift of Dr J Bergelson) or pcDNA3.1 expression vector alone (Invitrogen, Karlsruhe, Germany) using Effectene (Quiagen).
Highly purified native hBChE, anti-hBChE polyclonal antibodies, echothiophate (ETP) iodide [2- dithoxyphosphinyl thio- N, N, N-trimethylethanaminium iodide], and the wild-type hBChE cDNA construct in pRC/CMV encoding the full-length wild-type hBChE G117H single mutant were generously provided by O. Lockridge (University of Nebraska Medical Center, Omaha, NE).
Primers were designed to amplify the full sequencing construct in a single PCR reaction.
For the cells transfected with wild type FLAG-CHMP4B fulengthgth construct, in 553 cells (84.9%) there was colocalization between FLAG-CHMP4B and micronuclei or chromosome bridges, whereas for the mutant, 247 transfected cells (43.33%) showed colocalization of CHMP4B cataract mutant construct with micronuclei or chromosome bridges.
