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Amplified gene flanks are then mixed with specifically designed marker and binary vectors and treated with BP clonase, generating the deletion construct in a single cloning step.
Primers were designed to amplify the full sequencing construct in a single PCR reaction.
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In contrast, a whole mevalonate pathway constructed in a single operon was found to be less efficient.
Using this process, a single house or a colony of houses, each with possibly a different design, may be automatically constructed in a single run, imbedded in each house all the conduits for electrical, plumbing and air-conditioning.
The other terminus at Stockwell was also constructed in a single tunnel but with tracks on either side of a central platform.
According to the 13th century Chinese traveler Daguan Zhou, it was believed by some that the temple was constructed in a single night by a divine architect.
In other words, G(V E) has a vertex that is contained in all elementary circuits, and hence the characteristic c κ1) can be constructed in a single circuit-closing step.
We reported the development of concise total syntheses of - and ent--vindoline enlisting a tandem intramolecular [4 + 2]/[3 + 2] cycloaddition cascade of 1,3,4-oxadiazoles in which the fully functionalized pentacyclic ring system is constructed in a single step.
Importantly, the use of commodity hardware allows for significant flexibility: for example, we anticipate that this system (hardware and software) could be constructed in a single day assuming each of the work-up components was available as a pre-built unit.
To ensure that CL3EcoR cells were sufficiently sensitive to identify a single shRNAmir construct in a pool of 200 shRNAmir constructs, pRSp21F (a p21CIP1 shRNA construct) [ 17] was mixed in a ratio of 1 200 with pRSLaminA/C and used to assay bypass of senescence in CL3EcoR cells.
Our study does not support cross-country comparability with respect to working conditions of teachers with the result that certain aspects of job motivation are not part of a construct in single countries from an empirical perspective.
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