Separately, neither variation had a significant impact on protein function in the TA assay, but their presence in the same construct significantly impaired TA levels of the BRCA1 protein (Supplementary Figure S1).
In contrast to wildtype and T400A/T404A/S408A-mutated occludin, a phospho-mimetic Occ-T400E/T404E/S408E construct was impaired in binding to ZO-2.
As result, single-stranded gaps, which are inefficiently repaired by the host, are introduced in the final construct, thus impairing cloning efficiency.
Proposed in 1988 by Reaven (1), the construct that impaired glucose regulation and insulin resistance to glucose stimulation frequently coexist with elevated blood pressure and dyslipidemia has been evolving into several diagnostic criteria of metabolic syndrome (2– 4), a well-established risk for development of cardiovascular events and type 2 diabetes.
Finally, expression of the untagged aggregation-prone constructs impaired neurite growth (Fig. 12).
Expression of S405/418 phosphorylation-null constructs impaired carcinoma motility and adhesion, and also inhibited lamellipodia persistence monitored by live cell imaging.
We found that the virK mutant that we constructed was impaired for the ability to enter cultured epithelial cells and that virK expressed from a plasmid could restore invasion to near wild-type levels.
Accordingly, the mass transport of oxygen into the construct may be impaired, leading to increased hypoxia in the bioreactor system, compared with constructs cultured under free-swelling conditions.
Evidence for impaired construct validity was sought in a hierarchical regression analysis by investigating how much of the participants' FS-score variance was explained by the DKO-score, compared to the contribution of the knowledge level (NR-score), while controlling for Group, Gender, and Training length.
