Sentence examples for construct harbouring from inspiring English sources

The template used for epPCR was the pET28a-celstrep construct harbouring the wild-type gene of CelStrep previously described (Amore et al. 2012).

Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins.

A dual luciferase bicistronic construct harbouring the FGF-IA IRES was kindly provided by Dr. A.C. Prats [20].

In this study, we have demonstrated that a construct harbouring a duplicate of pre-microRNA miR-328 can be successfully expressed and processed to mature microRNA.

We then transfected BGC-823 cells with a reporter construct harbouring a core COX2 promoter sequence region (−327±59).

Gene silencing by targeting of the 3′UTR region of Prnp enabled the reconstitution of PrPc expression with a construct harbouring the Prnp open-reading frame.

DN-CHOP construct harbours mutations in the leucine zipper domain (L134A/L141A) that prevents its transcriptional activity [37].

Briefly, transgenic mice in the C57BL6 strain background were generated in which the gene construct harbours the human CBS with the mis-sense mutation 833 T>C.

Transfecting oocytes with GDF9 promoter deletion constructs harbouring the NBE reporter gene revealed a 33% increase in GFP as well as the luciferase signal signifying its role in stimulating the minimal promoter activity of GDF9 in buffalo oocytes.

In order to monitor the effectiveness of the drug treatments, transient transfections of luciferase reporter constructs harbouring the hormonal response region of the mouse mammary tumor virus promoter [54] were performed.

In agreement with this, we show that PDN treatment of cells transfected with monocistronic reporter constructs harbouring the utrophin A 5'UTR, causes an increase in reporter protein expression while leaving levels of reporter mRNAs unchanged.