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More details on construct generation can be supplied upon request.
Primers used for construct generation and site-directed mutagenesis were described earlier.
CJD: RT-PCR, CLSM analyses, generation and characterization of transgenic rice lines; OVP: construct generation, CLSM analyses; K JD: project design and supervision; DG: construct generation, RT-PCR, project design and supervision.
Construct generation, in vitro transcription and translation and protein expression and purification are described in [ 8, 27, 28].
In 2010, Weck and co-workers developed a method to construct generation 2 poly amide -based dendrons and dendrimer materials using click chemistry.
Having established that the Hsp68/Venus Hprt targeting reporter gene constructs offer a powerful alternative to study tissue-specific enhancer activity, we next sought to improve the efficiency of construct generation.
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Karl Mannheim's (1952) seminal work on historical generations and Norman Ryder's (1965) on cohorts serve as foundation for the historical cohort thesis.4 Mannheim's concept of socially constructed generations formed during late adolescence and early adulthood is pivotal for understanding how premigration experiences shape long-lasting views on life.
In this approach, to construct each generation the PDF (mathcal {P}) is used.
The construct for generation of transgenic plants described above was used as the effector vector for transient expression assay.
Nudity, meanwhile, is a construct my generation feels compelled by French theory to deconstruct, without pausing to consider that it might be useful.
RFLP markers have been used to construct first generation genetic maps [ 1, 2], but such hybridization-based markers have practical disadvantages.
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