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A HIS-fused construct for expression in bacteria of PC-1 C-tail (aa4132-4303) was generated and expressed in E. coli (BL21 strain).
A Pub1-mCherry-expressing strain lacking Nrp1 (Δ nrp1 ) was transformed with a construct for expression of GFP-µNS fused to full-length Nrp1, Nrp1PLD, or Nrp1RBD.
This difference may be explained by their use of a bicistronic construct for expression of the HLA-DM heterodimer; however, this strategy was also used to express HLA-DR, which still induced retention [8].
Construct for expression of CD4-anchored sFRP-3-HA protein was prepared as following.
Only two sense-oriented viral genes (V1 and V2) need to be added to the IR-carrying construct for expression and movement.
> -wrap-foot> In generating the N118-82Q mice, we employed a new technology in which we co-injected the htt transgene construct (PrP-N118-82Q) with a construct for expression of eGFP in skin (K14-eGFP).
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A vector was constructed for expression of Arabidopsis thaliana mitochondrial pyruvate dehydrogenase (E1) in the cytoplasm of Trichoplusia ni cells.
The plasmid pMS30 was constructed for expression of EGFP under a synthetic late promoter followed by an IRES cassette for expression of the puromycin acetyltransferase gene from the same transcript.
A series of plasmids was constructed for expression of eqFP611 in plants and for simultaneous expression of this fluorescent protein together with GFP.
Additionally, receiver operator characteristic (ROC) curves were constructed for expression of CIAPIN1 as a predictor for 'patient death' and 'cancer local recurrence'.
A recombinant modified pFastbac vector encoding the cytoplasmic domain of mouse Pcdh15-CD1 (GenBAccessionsioNo.o. NP_001165405) was constructed for expression in Sf9 insect cells.
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