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We have designed and tested a conditional miRNA-expression construct and showed that this construct expresses both the marker genes strongly and can silence the target gene efficiently upon Cre-mediated induction of the miRNA expression.
Each construct expresses a full-length RPS19.
The E1A construct expresses p300 protein with functionally inactivated histone acetyltransferase activity [24].
The K13-ERTAM construct expresses the K13 cDNA in-frame with the ligand-binding domain of a mutated estrogen receptor.
Because the HIC construct expresses the full-length HIC protein, we expected that like HIC it would activate transcription from the HIV-1 promoter.
This construct expresses separate CD44s and luciferase proteins.
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We generated the pBabe retrovirus construct expressing human SMAD4 to restore SMAD4 gene expression in SMAD4-deficient PDAC cell lines.
Transient expression of dominant-negative FGFR3 was achieved from an adenoviral construct expressing KD3 (KD3-IIIcv).
Mouse embryos carrying this construct expressed lacZ in their limb flexor and extensor tendons at E14.5.
A construct expressing such truncated protein resulted in significant AAS activity with decanoic, lauric and myristic acid (Fig. 2a, b).
Each construct expressed soluble protein in bacteria when expressed alone or in combination (Fig S3A).
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