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Moreover, immunohistostaining revealed that some of the adipocytes on the type I collagen construct expressed GFP. PPAR-γ2 gene expression in the induced ASCs in the type I collagen sponge was observed.
Since the SncA-PmpA fusion construct expressed using the same GlaA promoter in identical expression cassettes, the majority of SncA-PmpA will be localised to peroxisomes as well, this is shown in figure 4).
It is not clear why the CHO-B33 line should have produced so much more IgG than the others, but variation in yield depending on the construct expressed is a common finding both in this expression system and in others (discussed in [ 22]).
Mouse embryos carrying this construct expressed lacZ in their limb flexor and extensor tendons at E14.5.
Although the designed AurB69 333 construct expressed at high levels in Escherichia coli, the purified protein was largely unstable and prone to aggregation.
The hUCMSC inside the CPC + 0.1% RGD construct expressed the genes alkaline phosphatase, osteocalcin and collagen I, at twice the level of the CPC control.
The plasmid DNA construct expressed both human ZP3 and ZP4 epitopes, as revealed by transient transfection of COS-1 (African green monkey, kidney) mammalian cells.
The N-terminal deletion resulted in a mixed population of 30 and 55 nm VLPs, which differs from the same construct expressed in Escherichia coli, whereas pentamer aggregates resulted from deletion of the 428 465 region or the C428G mutation.
Each construct expressed soluble protein in bacteria when expressed alone or in combination (Fig S3A).
A GFP-tagged DISC1 construct expressed in NIH3T3 cells and rat striatal neurons localized near the base of primary cilia.
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Transient expression of dominant-negative FGFR3 was achieved from an adenoviral construct expressing KD3 (KD3-IIIcv).
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