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Similarly, flies expressing the translated CUG repeat construct express both polyleucine peptide and hairpin-forming RNA.
Adherent 4 T1-Luc mammaryammary carcinoma cells, transfected with Oct3/4 pGreenZeo promoter reporter construct (express green fluorescent protein zsGreen under the stem cell marker Oct-3/4 promoter), were grown as mammospheres, as mentioned before.
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We generated the pBabe retrovirus construct expressing human SMAD4 to restore SMAD4 gene expression in SMAD4-deficient PDAC cell lines.
Transient expression of dominant-negative FGFR3 was achieved from an adenoviral construct expressing KD3 (KD3-IIIcv).
Mouse embryos carrying this construct expressed lacZ in their limb flexor and extensor tendons at E14.5.
A construct expressing such truncated protein resulted in significant AAS activity with decanoic, lauric and myristic acid (Fig. 2a, b).
Each construct expressed soluble protein in bacteria when expressed alone or in combination (Fig S3A).
As a control, a similar construct expressing a shRNA targeting the luciferase transcript was used (Ctrl).
A pBABEpuro-βGalactosidase construct expressing β-Galactosidase served as vector control.
It was linked with the construct expressing versican G3 domain [54], producing the G3-UTR.
As a control, a separate construct expressing GFP alone (AdGFP) was used.
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