In late 2006, the Medicago genome sequence consortium (MGSC) constructed a partial genome assembly based on 1,996 Bacterial Artificial Chromosome (BAC) clone sequences as a basis for constructing draft pseudochromosomes.
Then, contigs were aligned with the reference genome to construct the draft chloroplast genome of each species in Geneious 9.0.5 software (http://www.geneious.com).
In an effort to strengthen duckweed research, we employed the Illumina high-throughput sequencing technology to construct a draft genome of L. minor (Fig. 1a).
Parameters of presence/absence of necrotic spots and spore concentration were exploited to construct a draft QTL map for comparison between the two isolates.
An in-house program was developed using BLASTX algorithms by comparing with five green algal lineages and one high plant species to construct the draft transcriptomic database of D. tertiolecta for RNA-Seq analysis and further target gene manipulation.
Newer methods such as described by Pop et al. [ 31] eliminate the overlap identification step in favor of moving directly to the creation of the multiple sequence alignment, thus reducing the amount of time required to construct a draft assembly considerably.
We take advantage of the close relationship between this species and the well characterized chicken (Gallus gallus; both belonging to the order Galliformes with a divergence time of 30 ~ 40 myr) [ 33– 35] and develop a reference guided assembly pipeline to construct a draft genomic sequence.
It may be said this constitutes as an elective monarchy…But by making the executive subject to impeachment, the term 'monarchy' cannot apply…" During the convention, Hamilton constructed a draft for the Constitution based on the convention debates, but he never presented it.
He constructed first draft and over-saw its completion.
The research team then constructed a draft measure which was revised by two Expert Panels of service users.
