In contrast, either administration of Shh or overexpression of Gli2 or gain-of-function PC1 C-tail construct down-regulates PPARγ2 promoter activity and expression, consistent with an inhibitory function of Gli2 hedgehog signaling on adipogenesis and fat formation [22], [23], [62], [63], [64].
Darcey Bussell, so easy and fresh-looking on stage, was a bundle of seemingly cranky habits before and after, swaddling herself like a walking duvet in specially constructed down-padded garments, in order to stop her leg and back muscles painfully seizing up.
We constructed another lentiviral vector (sh-TCF3 (lentiviral construct for down-regulation of TCF3)) to induce a stable knockdown of TCF3 in PDLSCs (Supplementary Materials).
They're constructed down to the hinges.
The GST-α5 construct pulled down small amounts of HA tagged kermit2.
A luciferase construct showed down-regulation of the PDCD4 promoter activity upon overexpression of miR-21, which is due to the direct targeting of the 3′UTR [16].
To understand the complex of this phylogenetically related gene family in the rice innate immunity, we designed a large inverted repeat construct to knock-down expression of all four members.
Utilisation of the recombinant PEX14N construct for pull-down from cytosolic fraction co-isolated PEX7 and the degradation pattern of PEX5, but not thiolase PTS2 cargo-protein (Fig. 4C).
It had been constructed upside down and a mobile crane was used to turn it over and place the windshaft into position.
When I was a college student at UC Berkeley I was adamant that my generation was going to turn the construct of gender upside down.
But in the end that is a bracing reminder that it was a real man, and not some fictional construct, who sat down opposite actual maniacs like Ed Kemper and David Berkowitz, and tried his level best to understand them.
