Exact(9)
SEM observations of the cell scaffold construct confirmed the tight attachment of chondrocytes to CG-PLLA NF and in-growth of cells into the interior of the membrane with proper maintenance of cell morphology.
Sequence analysis performed on each construct confirmed the intended point mutations at specific sites in GSK3ß S9A (data not shown).
D28472) was amplified by polymerase chain reaction (PCR) and the product was directionally cloned into the mammalian expression vector gWIZ3.0 (Gene Therapy Systems, Cambridge, UK) at the Hind III and Bam H1 restriction sites and the orientation of the construct confirmed by automated DNA sequencing.
Transient expression of the HlBCAT1-GFP fusion construct confirmed its mitochondrial targeting.
Control of transfection using a GFP construct confirmed the mesodermal distribution of the electroporated genes (Fig 4B, D).
Individual Ap-sensitive colonies were selected and integration of the gene construct confirmed by PCR and DNA sequencing (Secugen SL, Madrid, Spain).
Similar(51)
Direct sequencing of the constructs confirmed successful cloning.
All constructs confirmed to be the sequence of interest by sequencing.
Sequencing of the final constructs confirmed each desired mutation and revealed no other mutations.
The correct construct was confirmed by DNA sequencing (Fig. 1).
The pITF construct was confirmed by sequencing.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com