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Many markers of intestinal cell identity and genetic construct combinations have been developed for studies of intestinal homeostasis.
Both construct combinations were then delivered via Agrobacterium into Nicotiana benthamiana [ 18, 22] to test for the best possible combination of expression of the Id (data not shown).
In this paper we report 38 transgenic lines from two genotypes with different gliadin fractions silenced by RNAi, using three different promoter combinations and three different silencing construct combinations.
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A single colony of each strain and construct combination was grown overnight in 5 ml -Ura medium as a pre-culture.
Only one leaf per plant was used and identified to represent the same developmental stage (approximately 7 cm in diameter), and three biological replicates were used per construct combination.
We have explored the construction options in this report and found the most time-efficient solution for constructing combinations of any number.
By constructing combinations of double and triple mutants in the two adenylate cyclase genes (cyaA and cyaB), fimL, and cpdA, we show that ΔfimL mutants resemble ΔcyaB mutants in TM defects, decreased T3SS transcription, and decreased cAMP levels.
Our cloning strategy was verified by constructing combinations of up to 11 hairpin expression cassettes from both PCR and sub-cloned donor fragments, which as far as is known, is the largest reported combination (the largest outside of this study being 6) [7].
From their promoter sequence and from the phylogenetic evolution of these ANT genes in mammals, we constructed combinations of specific regulatory elements.
It consisted of a skillfully constructed combination of a broad historic spectrum, a moral worldview, experiences from the field, information, emotion, warmth, focused criticism.
Indoor room environment has a feature that is constructed combination of orthogonal planes each other, like floor, ceiling and walls.
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