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The resulting DNA construct coding for a 46 kDa HM was inserted into an expression vector and expressed as hexahistidine tagged fusion protein in Escherichia coli.
A gene construct coding for a VP2-GFP fusion protein was targeted to a pre-characterized high recombination efficiency locus flanked by flipase (Flp) recognition target sites and, after three weeks in selection, an isogenic cell population was obtained.
Presence or absence of indel polymorphisms was used to construct coding region haplotypes (Figure 2).
To test this hypothesis, we made a construct coding for a non-HA tagged version of the 38 aa TLR4 transmembrane domain.
The virus carried either a double-floxed-inverted Channelrhodopsin2-mCherry construct under control of the Ef1a promoter (CINs) or non-conditional construct coding for Channelrhodopsin2-mCherry under the synapsin promoter (corticostriatal) [34].
To evaluate the dimerization capability of zFoxp3, we designed a pull-down assay in which we co-transfected a plasmid coding for a His-tagged zFoxp3 with a construct coding for Foxp3 fused to Renilla luciferase (Ren).
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The final construct coded for a cleavable (enterokinase) N-terminal fusion with a maltose binding protein (MBP) and a non-cleavable C-terminal 10×His affinity tag.
The fusion construct coded for the IFBD2 (bp 6980 – bp 8142 GenBank Accession NM134448.2), an isoform b specific region just downstream of the plakin domain.
For this approach, an artificial gene is constructed coding for a protein of concatenated proteotypic peptides.
Construction of the coding frame followed best practice [ 38, 39]: two researchers (LS & PS) independently constructed coding frames based on a purposive sample of five transcripts from each site including patients with different demographic characteristics (age, gender, location of home).
Huffman codes use a static model and construct codes like that illustrated earlier in the four-letter alphabet.
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