Cells expressing the empty vector PCI NEO construct behaved as the parental WT cells (Fig. 4A).
The dynamics of the p53-Venus construct that we used have been extensively studied previously in MCF7 cells, which all showed that the reporter construct behaved similarly to the endogenous p53 [ 9, 10].
With the view that the final synthetic construct behaves as a cell factory, we analyse the helper functions that allow it to fulfil its production human-designed goal.
It is hypothesized that each theory-derived construct behaves differently in terms of their relationship with CVC.
Our data indicate that both fluorescently- and myc-tagged reporter constructs behave identically and exhibit a type-II membrane topology.
At 6 and 11 s, all RNA constructs behave similarly, and the normalized SHAPE changes indicate that Rev binding at Region1 and Region2 are mostly completed at those time points.
[ 10] This finding may indicate that despite their use in a malignant context, the ecdysone constructs behave with similar kinetics to that in normal cells thus implying a general utility in studies of various histological tumor types.
These results provide strong evidence that the presence of the linker does not cause major functional perturbations and that the tandem constructs behave essentially the same as the nonlinked proteins.
The constructs behaved as hydrogels and displayed stimuli-responsive characteristics towards temperature and salt concentrations.
