To further exclude that this phenotype is due to the insertion site of the attP2-integration sequence in the 5′ region of the Mocs1 gene41, we have generated a new R83E12-Gal4 construct and inserted it in the 86Fb landing site42, R83E12-Gal486Fb (Supplementary Fig. 2f).
Briefly, STING mutants were amplified by PCR or overlap PCR from HA-STING construct, and inserted into pCMV-HA vector with a HA tag at the N-terminus of each STING mutant.
Recombination sites from different site-specific recombination systems should be built as a unit into a construct and inserted into the same genomic site for direct recombination-efficiency evaluation, thus avoiding genomic position effects in the comparisons.
In the present study, a homology model of the human FFAR1 was constructed and inserted into a pre-equilibrated DPPC/TIP3P membrane system.
An artificial intron containing the insect cell polyhedrin (polh) promoter was designed, constructed, and inserted into the adeno-associated virus (AAV) Rep and Cap coding sequences to express the Rep and Cap proteins, respectively.
In order to carry out the neutronic performance evaluation, the neutronic models of the LLCB TBM set along with TBM frame have been constructed and inserted in the equatorial port of ITER reference neutronic model C-lite.
A second-generation CAR, consisting of an HER2-specific scFv linked to a hinge domain, a transmembrane (TM) domain, and CD137ζ and CD3ζ moieties in sequence (Fig. 1A), was constructed and inserted into a pseudotyped, clinical-grade lentiviral vector system.
The Cauliflower mosaic virus (CaMV) 35S promoter (35S), the ribozyme (RZ) sequence, and the nopaline synthase terminator (nos) were constructed and inserted between the left border (LB) and right border (RB) on pBD003.
