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Ten to 15 independent transgenic lines were produced for each combination of construct and host plant.
However, yields of plant-expressed AChE were modest because of limitations in the prototype expression construct and host plant [ 20, 21].
To determine if CiaD is delivered to the cytosol of human epithelial cells, INT 407 human intestinal cells were infected with C. jejuni transformed with the CiaD-ACD construct and host cell cAMP levels were measured via ELISA as described previously [ 12].
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Data from the program was uploaded to a secure website constructed and hosted by MCRI as well as being encrypted and stored on the mobile phones.
Data collected by the mobiletype program (for both intervention and comparison groups) on the mobile phone was sent via SMS to a secure website constructed and hosted by MCRI, where it was automatically collated and available for viewing.
Data collected by the mobiletype program (intervention and comparison) on the mobile phone were sent via SMS to a secure website constructed and hosted by MCRI, where it was automatically collated.
Recent attempts to construct and improve a model host for the heterologous expression of genes encoding secondary metabolites have done so by deleting nonessential genes [ 9, 10].
Though promising advances have been made with a variety of transgene constructs and host organisms, an exact mimic of a native dragline silk fiber has yet to be produced [e.g. 30], [33] [35].
It is difficult to compare the present observation with the results of previews studies, since the studies published so far on engineered secretion in lactic acid bacteria differ with respect to the genetic constructs and host strains used [ 42].
The headquarters of Banca Transilvania, at the intersection of Regele Ferdinand Avenue and Barițiu Street, is also a large contemporary building and was originally constructed to host the regional offices of Romtelecom, the public phone company, but was later sold to the bank.
A website (www.downyourdrink.org.uk) was constructed to host the DYD intervention and other components required for the trial.
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