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The mammalian retroviral vector pBABE-puro was used to construct and express full-length murine sFRP-3 and Wnt-1.
The reagents we used to construct and express ZFNs in our experiments are part of the OPEN Zinc Finger Consortium platform for engineering zinc finger arrays.
Qualitative studies are necessary in order to explore how people perceive, construct and express depression (see, for example, [ 7- 12]).
This study aimed to construct and express porcine EGF (pEGF), and use L. lactis as vehicle for producing and delivering pEGF.
The present study was aimed to construct and express the multifunctional staphylokinase variant SRH in the osmotically inducible E. coli GJ1158.
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HEK293T/17 cells were transfected with the Fab expression construct and the light chain construct and expressed Fabs purified from culture supernatant using HisTrap™ FF Columns (GE Healthcare).
The Aspergillus niger GOx was cloned into a pYES2-αMF-GOx construct and expressed extracellularly in yeast Saccharomyces cerevisiae.
Reconstitution of RAG1−/− mice with the EGFP+ Pax5−/− pro-B cells resulted in a T cell pool containing integrated retroviral construct and expressing EGFP.
To test for safety, we developed a baculovirus construct and expressed the rCB11-A9 protein in drosophila cells, because insect cells express a modest activation of lysine hydroxylase and hydroxylysine glycosyltransferase, allowing partial glycosylation of the product.
Various SAK-based multifunctional recombinant proteins were constructed and expressed in different expression system in earlier studies to reduce the reocclusion and bleeding complications.
In an effort to realize the soluble and functional OGT expression, the truncated mOGT recombinant (C-654) was constructed and expressed.
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