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The Appendix lists the items from each survey construct, along with their mean and standard deviation of responses.
Agrobacterium tumefaciens strain harboring each construct along with the p19 strain were infiltrated into 4-week-old N. benthamiana leaves.
The relative expression of E6 mRNA obtained by qRT-PCR in C33A cells after co-transfection with synthetically E6 construct along with miRNAs vectors or empty vector.
As shown in Fig. 3, transfection with synthetically E6 construct (E6) or along with empty pSilencer4.1-CMV vector (vector + E6) result in stable expression of E6 mRNA, while transfection with E6 construct along with miR-875 (miR-875 + E6) or miR-3144 (miR-875 + E6) individually decreased the expression of E6 mRNA to 53% or 42%.
HeLa cells were transfected with NF-κB luciferase reporter construct along with or without optineurin expression plasmids.
AB1157 or UNCNOMFD cells were transformed with pRCBCAT1 [20] construct along with pTrcmfd or pTrc99A, grown at 37°C in 2XYT medium.
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Since construction two new interchanges have been constructed, along with the expansion of the US 50 cloverleaf.
New stadiums were constructed along with new roads leading to the stadiums, construction that helped create thousands of jobs.
A new high-speed train line would also be constructed, along with pipelines for gas and oil.
A car barn and maintenance facility must be constructed along with three brick power substations to power the trolleys.
Its mixed urban and suburban landscapes now include business and shopping centres constructed along with high-rise office and residential buildings.
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