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Strains containing the URA3-yEGFP reporter construct adjacent to a terminal truncation of VIIL were created by transformation of FYBL1-8B [ 47] with pFEP41 digested with EcoRI and SalI.
Strains containing the URA3-yEGFP reporter construct adjacent to the core X of XIL and IIIR were obtained by transformation of FYBL1-8B [ 47] with the plasmid pFEP43 digested with SacI.
All yeast strains were derived from the isogenic S288c strains FY833, 1679-13B, DS288c3-1andanDS288C3-39c9c [ 46] or FYBL1-8B [ 47] and are listed in Additional File 5. Strains containing the core X mutations and the URA3 reporter construct adjacent to the core X of XIL were created as described previously [ 17].
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Soon, the campus will also have a dedicated music building, to be constructed adjacent to Kresge Auditorium.
It was not until early 1943 that crematoria was constructed adjacent to the gas chambers.
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It will be constructed adjacent to Kresge Auditorium, which has served for decades as the primary performance facility for MIT Music and Theater Arts productions and for student arts organizations.
While the number of female members of the house - there are currently 72 - has grown through the years, this issue was never addressed, even as a women's restroom was constructed adjacent to the Senate chamber in 1993.
However, the extension did not share the same success as the original channel because it was constructed adjacent to the Salmon River, which is supplied by glacial meltwater.
Rooftop solar will be installed on most of Union Point's downtown buildings, and a solar farm will be constructed adjacent to the development.
A Holiday Inn was constructed adjacent to the Big Sandy Superstore Arena between 8th and 9th Street in 1998.
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