Sentence examples for construct accession from inspiring English sources
We used the rat GluN1a splice variant construct (accession number NP_058706, hereafter referred to as GluN1) with a deletion from M848 to S938 [31], the rat GluN2A construct (NP_036705) with a deletion from I867 to V1464, and the rat GluN2B construct (NP_036706) with a deletion from I868 to V1482 [32].
The rat (r TRPV6 construct (accession # AF160798, kindly provided by M. Hediger, University of Berne, Switzerland) was used.
The 14-3-3σ 14-3-3σ 14-3-3σ aconstructNo. AFO29082) was made by PCR amplification of 14-3-3σ cDNA and sub-cloninGeneto the pGEM Eaccessionr (Promega, WI).
Briefly, SH-SY5Y cells were transfected with the appropriate pcDNA3.1/human HO-1 construct (Genbank Accession No.: NM_002133.2) using electroporation (Amaxa - Lonza, Slough, UK) according to manufacturer's instructions.
For this purpose, we used a matrix which we had recently constructed (TRANSFAC accession no. M00778; id V$AHR_Q5) [ 17].
The PCR product was digested using NcoI and HindIII, and then cloned into the NcoI and HindIII sites of pHA1011 (NCBI/DDBJ accession number LC016840) between the promoter and terminator regions of PeRBCS to construct pUT2 (NCBI/DDBJ accession number LC016842).
We constructed the accessions' phylogenetic tree using a Neighbor-Joining approach with 100 bootstraps.
The full-length human Brachyury construct (pCMV-Neo-Brachyury, accession number NM_003181.2), a p21 expression vector (accession number NM_078467.1) and empty-vector control (pCMV-Neo) were purchased from Origene Technologies Inc. (Rockville, MD, USA).
These reads have been deposited in NCBI's Sequence Read Archive along with the 454 reads used to construct the transcriptome under Accession number SRP018876.
The potential of these markers was assessed to detect intra-specific polymorphism and genetic diversity among desi, kabuli and wild accessions, and construct high-density inter-specific genetic linkage map of chickpea.
Considering labor costs and difficulties in amplifying and sequencing fragments greater than 2 kb, a smaller region covering only introns 2 and 3 (about 650 bp) of Adh1 (reduced Adh1) was used in all accessions to construct a phylogeny.
