To selectively trace connectivity of our in-vivo-reprogrammed neurons, we constructed a FLEX tracing vector with a tricistronic construct encoding for nuclear GFP (ncGFP), tva receptor, and rabies GP under the synapsin promoter.
We have constructed a circuit-tracing PRV-Bartha derivative, PRV369, that expresses the fluorescent [Ca2+] indicator G-CaMP2.
Second, we need to construct a robust phylogenetic framework to trace the evolutionary pathway of each species with high confidence.
This strategy of combining small traces to construct a bigger one can be used in a sliding window algorithm.
We study recognition ability of humans and line trace programs in order to construct a reverse Turing test based on one-stroke symbol traces.
The similarity that enforces the study to adopt this method is that both the study and process tracing method seek to construct a phenomenon (regional cooperation on GBM) by testing an empirical model and by analyzing the historical chronicle of different explanations.
If you wanted to, you could perhaps construct a theory that the two films, placed together, trace America's postindustrial urban decline.
Using local snapshots, we avoid high dimensional representation of trace spaces and use some local features of the solution space in constructing a low dimensional trace space.
Viterbi [6] and Forney [11] showed that the min-sum algorithm can be used by constructing a trellis and tracing a path, corresponding to the most likely transmitted symbol/codeword sequence through the trellis.
