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Commercial (R -3-HB (Sigma-AldR -3-HBas used to constR -3-HBstandard curve.
(c) Linearity of the standard curve: Analyze each of the 5 standards, and construct a standard curve.
This shows that it is possible to use this method to construct a standard curve for the estimation of lipase solutions with unknown concentrations by measuring the intensity of its hydrolysis spot on polymer film.
Calibrated QuantiBRITE fluorescent beads were used to construct a standard curve for quantification of CD38.
This value was further used to construct a standard curve for ELISA (see infra).
Ten fold serial dilutions of cDNA from the untreated cell lines, from 1 to 1 100, was analysed and used to construct a standard curve for each gene.
RNA was eluted in 40 µl of RNAse-free H2O by centrifugation at 11,000 g for 1 min. Synthetic RNA transcripts for CHIKV and DENV-2 were generated to construct a standard curve.
DNA Matrix plasmid standards of known concentration from −5 (4.8×106) to −10 (4.8×101) were used to construct a standard curve, from which the genome copies in the samples could be calculated.
The signals from the internal standards were used to construct a standard curve, which was used to determine the genome copy number using the average signal from the 1 kbp genomic fragment.
The Ct values of the standards were used to construct a standard curve (compare Fig. 2C) that was used to quantitate the genome copy numbers in the cell lysates and to check the pcr efficiencies in the lysates including internal standards.
Collagen standard solution was utilized to construct a standard curve.
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