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The F2 mapping population of 94 individuals was genotyped at the loci tagged by the preselected anchor markers to construct a linkage map skeleton.
Two newly developed markers and two polymorphic SSR markers (RM17713 and RM13) were used to construct a linkage map of NUDA/GL-1 using Mapmaker/Exp 3.0 (Lander et al[1987]) on the basis of the 158-plant F2 population.
For linkage analyses, we selected 355 SNPs uniformly distributed from the original marker set to construct a linkage map using the Kosambi map function in R/qtl (Arends et al. 2010).
For the QTL analysis, 110 BILs were developed from BC2F2 plants produced by crossing Hitomebore (the recurrent parent) with Kele and were used to construct a linkage map and to search for QTLs for resistance to BGR.
An RIL population was simulated for 11 equally spaced markers that construct a linkage group of length 200 cM.
Nonetheless, despite the small number of individuals sampled we were able to construct a linkage map of the P. xylostella genome with paternally derived RAD alleles.
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Besides the linkage map construction with JOINMAP, we followed the procedures described in [26] for constructing a linkage map with MAPMAKER 3.0.
The former relations are obtained through constructing a linkage between the strain energy induced in the continuum and the potential energy stored in the atomic bonds using the Cauchy Born rule.
Having effectively exhausted the a priori list of promising melanisation candidates, we are currently in the process of constructing a linkage map of B. betularia to identify the region that controls this famous polymorphism.
We genotyped 61 progeny at 739 single nucleotide polymorphisms (SNP) spread throughout the genome, and constructed a linkage map that was largely consistent with the genomic sequence, with the exception of one potential inversion of ∼527 kb on Chromosome V.
We constructed a linkage map consisting of 834 markers in 22 linkage groups that spanned over 1,933 cM.
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