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The resulting high quality cDNAs were used to construct a library for paired-end sequencing.
The Enz DNA extraction was split into two samples (Figure 2), one of which was cloned and sequenced using Sanger sequencing while the other was used to construct a library for pyrosequencing.
The sRNAs were enriched from porcine exosomes to construct a library for Solexa sequencing.
The required fragments were purified by AMPure XP beads and enriched by PCR to construct a library for transcriptome sequencing.
The WGA DNA was used to construct a library for 454 sequencing, using the GS FLX Standard DNA Library Preparation Kit (Roche) according to the manufacturer's instructions.
The resulting cDNA was used to construct a "library" for sequencing by the addition of adaptors as per the supplier's instructions (Roche Diagnostics) and sequencing was conducted at Penn State University on an FLX model 454 DNA sequencer (454 Life Sciences) as previously described [ 32].
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In order to construct a library of interchangeable parts, further search for putative molecules behaving as automata is required.
They constructed a library of synthetic promoters for Lactococcus lactis that also covered 3 to 4 logs of promoter activity.
Thus, WTA is a necessary step to construct a cDNA library for single cell transcriptomic sequencing.
The PCR products were used to construct a clone library for each primer set with the pGEM-T Vector System cloning kit (Promega, Madison, WI).
To construct a PSSM library for transmembrane proteins, we first ran rps-BLAST against the CDD database, and consider, out of the alignments reported, only those PSSMs that we have previously annotated as transmembrane by keyword (CDD 539).
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