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This suggested that (1) RhoA activation was essential for the constriction of actomyosin rings after oocyte activation, and (2) while increased MPF activities activated RhoA possibly by inactivating GAPs during the assembly, decreased MPF activities activated RhoA probably by activating GEFs during the constriction of the actomyosin ring.
Together these mechanisms promote the successful constriction of the actomyosin ring prior to the initiation of mitosis.
While much is now known regarding the assembly and constriction of the actomyosin ring, our understanding of the mechanisms (if any) monitoring the completion of cytokinesis is lacking.
Therefore, the constriction of the actomyosin purse-string is not applicable to wound repair in Dictyostelium cells, and it is not a common mechanism for wound repair in eukaryotic cells.
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One force is generated by the circumferential constriction of epidermal actomyosin rings and was identified nearly 20 years ago as the major driver of embryonic elongation (Priess and Hirsh, 1986).
As described above, the embryo acquires an elongated shape through the circumferential constriction of ring-shaped actomyosin bundles arrayed along the surface of the epidermis (Priess and Hirsh, 1986).
The SIN defines a network of essential genes that are required for the constriction, but not the assembly, of the actomyosin ring [6] [8].
It assembles prior to any visible evidence of plasma membrane inward growth, which occurs concomitantly with the formation and constriction of a contractile actomyosin ring and synthesis of the septum wall.
These results have led to a model in which myosin enriches at the apical, contact-free cell cortex of endodermal precursors, and activation of myosin results in an actomyosin-dependent constriction of the apical surface of these cells, driving movement of the cells to the embryo interior (see R ohrschneider and N ance 2009; Sawyer et al. 2010 for review).
Here we present data to show that a mutation in the FERM-domain binding motif of the polarity determinant Crb affects major steps during DC, namely elongation of the DME cells, proper formation of the actomyosin cable at the LE, and regulated constriction of the AS cells.
- In fission yeast, just as in more developmentally complex eukaryotes, cytokinesis is achieved through the regulated assembly and subsequent constriction of a contractile actomyosin ring.
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