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At MCF10a : BT-474 ratios as high as 18 : 1 (maximum ratio tested due to constraints of the assay), the presence of 'normal tissue' had no significant impact on the ability of ALM to specifically bind to tumour cells.
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Constraints of the sequence context on assay performance meant we could not analyze all CG dinucleotides in the relevant Infinium probe, however at least one of the CG dinucleotides pyrosequenced was identical and the others were by definition adjacent.
Tissue transglutaminase (tTG) assay was available, but one-third of centers reported financial constraints in the regular purchase of the assay kits.
Therefore, it is not feasible with this method to correctly determine Ki values for inhibitors with binding affinities higher than the fluorescent ligand because of the assay constraint.
The simplification of tetraploid genotypes to a diploid model, the biallelic nature of the assay, assay design constraints, ascertainment bias, and sampling of a single individual from each wild Solanum species may have contributed to the differences in heterozygosity observed between wild species and cultivated potato.
Runtime of the assay was 20 minutes.
Management is assumed to pursue this objective, subject to the constraints of the input and output markets, technological constraints, legal (regulatory) constraints and ethical constraints.
That means working within the constraints of the existing infrastructure.
Q. Can you talk about the constraints of the budget?
"Art overcomes the constraints of the word.
Because of logistical constraints, many of the CV-1 assays with the site samples were performed only once.
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