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Between those two poles lies a realm of environmental and pragmatic considerations that reflect the goals, biases, risk-tolerance, and constraints of study sponsors and organizers.
The major limitations for application of these results to clinical practice are attributable to constraints of study design.
Unfortunately, we were unable to include patients who presented to the hospital outside of the daytime hours, due to the constraints of study personnel.
However, the difficulty of recruiting young minority participants for an intensive metabolic study, coupled with constraints of study budget and timeline, affected our final recruitment, as documented by Shahabi et al. (15).
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This was not possible in the current study due to constraints of the study design meaning that only three time points were available for analysis.
It is not possible, within the constraints of this study, to report on the relative efficacies of the different seeding cells in repairing bone defects, but the osteogenetic potential of allogenic tissue-engineered bone membrane in animals forms the basis for further study.
The first activity is to review the scope and constraints of the study design and to establish the study parameters.
Within the resource constraints of this study, it is unlikely that this study will be adequately powered to explore these interactions.
It was not feasible to invite these subjects to participate in the study during their second visit due to security constraints of the study setting faced by the researchers.
The STROBE statement (strengthening the reporting of observational studies in epidemiology) was followed, within the constraints of our study design.
Future work should circumvent the constraints of this study by including targeted sampling of the viral community (i.e., <0.1 μm fraction) for metagenomic assessment, as well as isolation/characterization, along with studies to determine viral host dynamics.
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